I have the sim on an external HDD drive, and i would like to test if there is any improvement by putting the SIM on the internal disk of the pc. If i copy and paste my whole FS2020 folder, will it give me a problem when trying running it from a different location? Dont want to find out the hard way…

If you go to Windows settings > Apps > Apps and features you have the option to move you sim. I never tried it but that’s how I would do it. I don’t think copy and paste would work.