I’ve had good luck taking off in the DC-3 since I started adjusting the trim before takeoff. It does not default to where you need it at takeoff.
You absolutely need to dial in some nose-down trim and some right-rudder trim or it will roll to the left and jump nose-up shortly after takeoff, leading to a stall or crash if you don’t immediately regain control.
The various DC-3 threads should include some recommended values (you can read off the indicator the numbers it’s near) but I just kinda “wing it” myself. 
Watch carefully how it’s responding, and keep very careful eye on your airspeed to make sure you’re not pitched up so much you’re heading for a stall.